Reyes-Lamothe Lab

McGill University

Selected Publications

  1. Beattie TR*, Kapadia N*, Nicolas E, Uphoff S, Wollman AJM, Leake M, and Reyes-Lamothe R (2017) Frequent exchange of the DNA polymerase during bacterial chromosome replication. eLife, doi: http://dx.doi.org/10.7554/eLife.21763
  2. Highlighted by Faculty of 1000

    In this paper we showed that most replisome subunits bind to the replisome for few seconds each time.

    In the image a single copy of the proofreading exonuclease of DNA Pol III binds and unbinds multiple times in the span of 1 minute.

  3. Beattie, TR & Reyes-Lamothe R (2015) Replisome’s journey through the bacterial chromosome. Frontiers in Microbiology 6: 562. doi: 10.3389/fmicb.2015.00
  4. Reyes-Lamothe R, Tran T, Meas D, Lee L, Li AM, Sherratt DJ, Tolmasky ME (2014). High-copy bacterial plasmids diffuse in the nucleoid-free space, replicate stochastically and are randomly partitioned at cell division. Nucleic Acids Res. 42, 1042-51.
  5. In this paper we showed that plasmids accumulate at the poles as they get excluded from the nucleoid. However, they can travel accross the cell body from time to time.

    In the image a plasmid can be observed traveling from midcell to one of the cell poles.

  6. Uphoff, S., Reyes-Lamothe, R., Garza de Leon, F., Sherratt, D.J., Kapanidis, A.N. (2013). Single-molecule DNA repair in live bacteria. Proc Natl Acad Sci U S A 110, 8063-8068.
  7. Highlighted by Faculty of 1000

  8. Badrinarayanan, A., Reyes-Lamothe, R., Uphoff, S., Leake, M.C., and Sherratt, D.J. (2012). In vivo architecture and action of bacterial structural maintenance of chromosome proteins. Science 338, 528-531.
  9. Here we studied the SMC-like complex, MukBEF, in live E. coli. Our single-molecule data showed that the active form is a dimer of complexes, we describe dynamic binding of these complexes and their dependency on ATPase activity.

    The plot shows the relation between the stoichiometry of the different subunits, demonstrating a fixed ratio between their numbers

  10. Badrinarayanan, A., Lesterlin, C., Reyes-Lamothe, R., and Sherratt, D. (2012). The Escherichia coli SMC Complex, MukBEF, Shapes Nucleoid Organization Independently of DNA Replication. J Bacteriol 194, 4669-4676.
  11. In this paper we showed that to the SMC-like of E. coli, MukBEF, does not require to work during the replication of the chromosome to shape it into the nucleoid.

    In the image, a cell without one of the subunits of MukBEF (with another subunit labeled in green) induces the expression of the missing subunit. The relocalization of a loci close to the replication origin can be observed happening from the pole to the midcell, where it is usually found.

  12. Reyes-Lamothe, R., Sherratt, D.J., and Leake, M.C. (2010). Stoichiometry and architecture of active DNA replication machinery in Escherichia coli. Science 328, 498-501.
  13. Highlighted by Faculty of 1000

    Here we determined the stoichiometry of the replisome subunits in live cells using single-molecule microscopy. Our data showed that there were on average 3 copies of the DNA Pol III at each replisome.

    This figure shows the principles of the techniques used in the paper. A special illumination technique, named slimfield, allowed us to collect pictures of cells carrying fluorescent fusions of the replisome at millisecond rates, similar to the time necessary for the DNA Pol III to incorporate a nucleotide. We used a technique called stepwise photobleaching to count the number of fluorescent molecules at each of the replisomes (observed a spots).

  14. Reyes-Lamothe, R., Possoz, C., Danilova, O., and Sherratt, D.J. (2008). Independent positioning and action of Escherichia coli replisomes in live cells. Cell 133, 90-102.
  15. Highlighted by Faculty of 1000

    Highlighted by Nature Reviews Molecular Cell Biology

    In this publication we describe the localization of DNA replication in actively growing E. coli. We demonstrated that the DNA replication machinery, called the replisome, does not need to group with other copies of itself to work –a popular model at the time for both eukaryotes and prokarytes. Instead, we show that the replisome dynamically migrates in the cell during DNA replication, most of the time occupying a space away from its sister replisome (originated at the same replication origin).

    The plots show the position of the replisome (green line) relative to the length of the cell over the cell cycle in a single cell. In addition, the position of two chromosomal loci, normally positioned at the poles of the nucleoid, is also shown.

For a complete list of publications click in here